اثر کافئین براستخوانسازی و سیستم اسکلتی

ABSTRACT The Purpose of this study was to identify the effect of caffeine on development of skeletal tissue and osteofication. Since osteofication in rat starts on day 13th, so we started the study on day 13th of pregnancy. A dose of 80mg/kg caffeine (1%) in the 13th ,14th and 15th day of pregnancy was injected to the test group intraperitoneally and a dose of 80mg/kg of Normal saline was injected to the control group in the same way in the 13th,14th and 15th day of pregnancy On the 16th to 19.5th day of pregnancy in every four hours a single group of test and control rats were sacrificed and their fetuses were separated from the pregnant rats by sacrification and their fetuses were separated from the pregnant rats and were fixed separately in Normal salin and 5 m sections were prepared. The study was completed by applying the following staining techniques: Feulgen ,toluidine blue ,P.A.S and H &E;The following results were obtained: 1-Caffeine causes 36 to 48 hours delay in osteofication. 2- Caffeine causes skeletal abnormalities in the test fetuses compared with control ones;These abnormalities include: a- Adactylia b- Syndactily C- Meromelia d- Microcephaly e- Hydrocephaly f- Micrognathia g- Clubfoot